Andrographolide Kill Mycrobacterium Tuberculosis

 Phatogenesis of tuberculosis based on the immunopathologic,as defect control immune response to M.tuberculosis infection.Interaction of macrophage with M.tuberculosis is the central to all stages of tuberculosis.Therefore the immunomodulator may have an important role in the intracellular killing activation of macrophages.

The aim of this research to prove the effect of andrographolide (AND1)- Sambiloto (Andrographis paniculata) on the macrophages intracellular killing activation to M.tuberculosis infection in vitro.Experiment study,one group of the macrophages culture from pheripheral blood of tuberculosis patients and healthy people with AND1 treatment are infected by M.tuberculosis ,to proveon intracellular killing activation effect of the macrophages;compare with control group wiyhout treatment.The macrophages intracellular killing measure by CFU (Colony Forming Unit) per ml.

Using T-test and ANOVA analysis showed that among tuberculosis patients macrophage cultures, pre and post AND1 treatment were significant different (p=0,00),there were after treatment decreasing of CFU/ml at 7 days incubation;on the other hands,among immunized healthy individuals macrophages culture ,pre and post treatment have CFU/ml significant difference at 72 hours and 72 days incubation (p=0,00 and p=0,001).

The result demonstrate that macrophages intracellular killing activation to M.tuberculosis with AND1 more effective than without AND1.Therefore the immunomodulator finding may important on tuberculosis therapy development.

Written by Prof.Dr.Ni Made Mertaniasih. dr.MS.Sp.MK